江西省农业微生物资源开发利用工程实验室

发布日期:2017-10-30浏览次数:


江西省农业微生物资源开发利用工程实验室(以下简称“实验室”)于2014年获批组建。实验室以生物活性为导向,运用传统与现代高通量筛选技术相结合,开展农业微生物资源的筛选与育种,建立生物农药、农业废弃物生物转化、农田污染物生物修复等领域的菌种资源库;针对农业微生物资源产业化开发体系,应用现代微生物学、分子生物学、发酵调控与生化工程的理论和技术,研究农业微生物产业领域的产品共性及关键技术的基础理论及机理,并通过引进、整合国内外农业微生物科研成果及技术,形成可适应不同规模和技术水平农业科技企业使用的农业微生物品质控制工艺流程,完善我省农业微生物产品检测技术体系,并面向省内外企业开放。

实验室现有主要研究人员21人。其中正高8人,副高7人,均具有博士学位。拥有国家级人才1人,省级人才9人,校级人才10人。近年来承担各类纵向和横向课题80项,包括了国家级科研项目32项,省级科研项目40项,其他项目8项。在SCI、EI、A类期刊上发表学术论文300余篇,国家授权发明专利20项,获江西省科技成果奖5项。主要研究领域包括:农业微生物资源开发与利用、环境污染物的微生物法降解与转化、合成生物学与代谢工程、动物肠道微生组学研究与利用、基因与酶功能解析及生物信息学开发利用等。

工程实验室包含农业微生物资源高通量筛选研究室、微生物理化与分子育种室、微生物活性物质评价与质量控制室、代谢调控及发酵工艺控制室、发酵产物分离制备室等等。拥有梯度PCR仪、荧光定量PCR仪、凝胶成像分析系统、全自动酶标仪、蛋白纯化系统、自动平板培养基分装系统、单细胞分离提取系统、倒置荧光显微镜、荧光显微镜、高效液相仪、气相色谱仪、各类培养箱与摇床、各种规格小型发酵罐、1000升全自动发酵罐以及多功能发酵产品提取中试线等仪器设备。


Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources

Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources (hereinafter referred to as "the Laboratory") was approved for establishment in 2014. The Laboratory is oriented towards bioactivity and combines traditional and modern high-throughput screening technologies to conduct screening and breeding of agricultural microbial resources. It aims to establish culture collections in areas such as biopesticides, bioconversion of agricultural waste, and bioremediation of farmland pollutants. Focusing on the industrialization development system of agricultural microbial resources, the Laboratory applies modern microbiology, molecular biology, fermentation regulation, and biochemical engineering theories and technologies. It studies the commonalities and key technologies of products in the agricultural microbial industry, as well as the fundamental theories and mechanisms involved. By integrating and introducing domestic and international research outcomes and technologies in agricultural microbiology, the Laboratory aims to develop quality control process flows that can be adapted for agricultural science and technology enterprises of various scales and technical levels. It also seeks to improve the agricultural microbial product testing technology system in the province and make it accessible to enterprises both within and outside the province.

The Laboratory currently has 21 main researchers, including 8 senior professionals and 7 associate senior professionals, all holding doctoral degrees. It boasts 1 national-level talent, 9 provincial-level talents, and 10 university-level talents. In recent years, it has undertaken 80 various vertical and horizontal projects, including 32 national-level research projects, 40 provincial-level research projects, and 8 other projects. The Laboratory has published over 300 academic papers in SCI, EI, and Class A journals, has been granted 20 national invention patents, and has received 5 science and technology achievement awards from Jiangxi Province.

The main research directions include the development and utilization of agricultural microbial resources, microbial degradation and transformation of environmental pollutants, synthetic biology and metabolic engineering, research and utilization of animal gut microbiomes, gene and enzyme function analysis, and bioinformatics development and utilization. The engineering laboratory comprises research facilities for high-throughput screening of agricultural microbial resources, physicochemical and molecular breeding, evaluation of microbial active substances and quality control, metabolic regulation and fermentation process control, and fermentation product separation and preparation.

The Laboratory is equipped with a gradient PCR instrument, fluorescent quantitative PCR instrument, gel imaging analysis system, fully automated enzyme labeling instrument, protein purification system, automated agar plate dispensing system, single-cell separation and extraction system, inverted fluorescence microscope, fluorescence microscope, high-performance liquid chromatography (HPLC) system, gas chromatography (GC) system, various types of incubators and shakers, small-scale fermentation tanks of various specifications, a 1000-liter fully automated fermentation tank, and a multifunctional pilot line for the extraction of fermentation products.